On the Fractionation of Agglutinins and Antitoxin
نویسنده
چکیده
E. P. Pick’ in rgor associated a number of antisubstances individually with the one or the other of the two serum globulin fractions of the Hofmeister classification. In the pseudoglobulin (3.4 to 4.6 saturated ammonium sulphate solution)2 group of antibodies he placed the diphtheria and tetanus antitoxins and the typhoid agglutinin of horse serum; the lower or euglobulin fraction (2 .g to 3.4 saturated) comprises diphtheria and tetanus antitoxin and cholera lysin in the goat, rabbit and guinea pig, and finally cholera agglutinin in the horse and goat. It becomes possible, according to Pick, to separate theindividualspecifically reacting antisubstances by fractioning appropriate mixtures of sera. Such a possibility suggested the application of this method to the further study of certain antibodies, especially of the relation of specific and group agglutinins developed by immunization against a single strain of organism. Preliminary experiments in the course of our investigation indicated the unreliability of Pick’s differentiation, and attention was accordingly directed to the actual possibility and practicability of distinguishing between antibodies by fractionation of the globulin. The availability of polyagglutinative sera for the work gave a chance for making numerous and extended observations of the distribution of these antibodies in the fractions3
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تاریخ انتشار 2003